fluoromax 4 user manual
LINK 1 ENTER SITE >>> Download PDF
LINK 2 ENTER SITE >>> Download PDF
File Name:fluoromax 4 user manual.pdf
Size: 4803 KB
Type: PDF, ePub, eBook
Category: Book
Uploaded: 4 May 2019, 20:45 PM
Rating: 4.6/5 from 556 votes.
Status: AVAILABLE
Last checked: 3 Minutes ago!
In order to read or download fluoromax 4 user manual ebook, you need to create a FREE account.
eBook includes PDF, ePub and Kindle version
✔ Register a free 1 month Trial Account.
✔ Download as many books as you like (Personal use)
✔ Cancel the membership at any time if not satisfied.
✔ Join Over 80000 Happy Readers
fluoromax 4 user manualAll rights reserved. No part of this work may be reproduced, stored, in a retrieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from HORIBA Instruments Incorporated. D (30 Jul 2012) 14: Bibliography.14-1 15: Compliance Information.15-1 Declaration of Conformity. 15-1 Supplementary Information.15-1 16: Index.16-1. D (30 Jul 2012) D (30 Jul 2012) Introduction Disclaimer By setting up or starting to use any HORIBA Instruments Incorporated product, you are accepting the following terms: You are responsible for understanding the information contained in this document. You should not rely on this information as absolute or all-encompassing;. D (30 Jul 2012) Introduction rated be held liable for any special, incidental, indirect or consequential damages of any kind, or any damages whatsoever resulting from loss of use, loss of data, or loss of profits, arising out of or in connection with our products or the use or possession there- of. D (30 Jul 2012) Introduction Safety summary The following general safety precautions must be observed during all phases of opera- tion of this instrument. Failure to comply with these precautions or with specific warn- ings elsewhere in this manual violates safety standards of design, manufacture and in- tended use of instrument. D (30 Jul 2012) Introduction Risk of electric shock. This symbol warns the user that un-insulated voltage within the unit may have sufficient magnitude to cause electric shock. Caution: Danger to fingers. This symbol warns the user that the equipment is heavy, and can crush or injure the hand if precautions are not taken. D (30 Jul 2012) Introduction Caution: This instrument is used in conjunction with ultra- violet light. Exposure to these radiations, even reflected or diffused, can result in serious, and sometimes irre- versible, eye and skin injuries.http://promkoop.ru/userfiles/financial-management-policy-and-procedures-manual.xml
- Tags:
- fluoromax 4 user manual, fluoromax 4 user manual, fluoromax 4 user manual pdf, fluoromax 4 user manual transmission, fluoromax 4 user manual user, fluoromax 4 user manual transfer switch.
D (30 Jul 2012) Introduction lens can also be damaged, but because the cornea acts as a filter, the chances are re- duced. This should not lessen the concern over lens damage however, because cata- racts are the direct result of lens damage. D (30 Jul 2012) Introduction Additional risks of xenon lamps Warning: Xenon lamps are dangerous. Please read the fol- Among the dangers associated with xenon lamps lowing precautions.Fire ignited by hot xenon lamp. D (30 Jul 2012) Introduction The skin and eyes absorb infrared radiation (IR) as heat. Workers normally notice ex- cessive exposure through heat sensation and pain. Infrared radiation in the IR-A that enters the human eye will reach (and can be focused upon) the sensitive cells of the ret- ina. D (30 Jul 2012) Introduction 0-12. Contact your Sales Repre- sentative or the Service Department for details. Caution: HORIBA Scientific is not liable for damage from line surges and voltage fluctuations. A surge protector is strongly recommended for minor pow- er fluctuations. For more severe voltage varia- tions, use a generator or uninterruptible power supply. If a host computer (PC) is ordered as a part of the system, the PC is delivered in a few clearly labeled boxes. Adjust the four leveling feet on the bottom of the instrument. Inspect for previously hidden damage. Notify the carrier and HORIBA Scientific if any is found. Check the packing list to verify that all components and accessories are present. If the computer is not from HORIBA Scientific, perform the installa- tion. Contact a HORIBA Scientific Sales Representative for recommended specifications for a suitable host computer. Choose the desired in- strument you wish to emulate. Click the OK button. The System Initialization Process window opens: Under the Status column, warning. D (30 Jul 2012) System Description 2: System Description Warning: Do not open the instrument without proper training, appropriate protection, and having read this operation manual.http://gdctogo.com/mae/mm/financial-management-manual-teqip.xml The in- strument contains dangerous voltages, ultra- violet, visible, and infrared radiation, and frag- ile light-sources. D (30 Jul 2012) System Description When setting slit width, the trade-off is intensity of signal versus spectral resolution. The wider the slits are, the more light falls on the sample and detector, but the resolu- tion decreases. See Chapter 8 for more details. Xe-lamp power supply (1a) Side-panel circuit board Optional phosphorimeter-. Note: HORIBA Scientific suggests leaving the lamp on during brief pe- riods of inactivity. D (30 Jul 2012) System Operation Turning on the system Warning: When the xenon lamp is ignited, a large voltage is ap- plied across the lamp. There- fore, never operate the lamp Turn on the with the cover removed. To be sure that your instrument is properly calibrated, call Fluorescence Service for assistance. We can arrange a visit and cali-. D (30 Jul 2012) System Operation Excitation-monochromator calibration check This calibration check verifies the wavelength calibration of your excitation monochromator, using the reference photodiode located before the sample compart- ment. It is an excitation scan of the xenon lamp’s output, and should be the first check performed. D (30 Jul 2012) System Operation Use the default parameters or adjust them.The main peak ought to be at 467 nm, but here appears near 480 nm. D (30 Jul 2012) System Operation Cursor Cursor’s wave- length in nm This example shows the peak actually at 477 nm, which is 10 nm too high. Therefore we must recalibrate the monochromator. Click the Previous Experiment button The Experiment Setup window appears. D (30 Jul 2012) System Operation to view the monochromators’ index card, Click the Monos icon then click the excitation monochromator tab:. D (30 Jul 2012) System Operation Enter the current, observed position (here, 477 nm) of the peak in the Position Control field, then hit the Enter key. Click the Calibrate Excitation 1 button.http://dev.pb-adcon.de/node/19778 The Calibrate window opens: In Peak Of Interest, enter. D (30 Jul 2012) System Operation Emission-monochromator calibration check Note: The emission-monochromator calibration of the instrument is di- rectly affected by the calibration of the excitation monochromator. This calibration check verifies the wavelength calibration of the emission monochromator with the emission photomultiplier tube. The water-Raman experiment automatically loads. Use the default parameters or adjust them. Monochromator parameters for the water-Raman scan: Monochromator (1200 Initial wave- Final wave-. Additionally, you may want to record the water-Raman intensity daily or weekly.D (30 Jul 2012) System Operation 3-16. D (30 Jul 2012) Data Acquisition Experiment Menu button The Experiment Menu button chooses an overall type of experiment to run, such as an emission scan, a phosphorimeter scan, a synchronous scan, etc., based on the instru- ment and connected accessories, such as a temperature bath, MicroMax, etc. D (30 Jul 2012) Data Acquisition Click the Experiment File field, and enter a new file name or select a previously saved file. Verify that experimental parameters are correct. Be sure to check all parameters under all icons in the left-hand column. Insert the sample into the sample compartment, and close the sample compartment’s cover. D (30 Jul 2012) Data Acquisition Previous Experiment Setup button The Previous Experiment Setup button resets the experiment to the previous experi- ment used, with minor modifications to the hardware possible. Note: The Previous Experiment Setup button is active only after an ex- periment already has been loaded. D (30 Jul 2012) Data Acquisition Auto Run Previous Experiment button The Auto Run Previous Experiment button reruns the last experiment loaded without modifications. Note: The Auto Run Previous Experiment button is active only after an experiment has already been loaded and run. An overlay file can be used to compare data in real time. Inclusion of an overlay file is con- trolled in the Display Options icon in the Experiment Setup window. D (30 Jul 2012) Data Acquisition 3D Scan to 3D Profile button The 3D Scan to 3D Profile button extracts emission profiles from an excitation- emission matrix. Open excitation-emission matrix data. Click the 3D Scan to 3D Profile button in the toolbar. D (30 Jul 2012) Data Acquisition Click the Arbitrary Line button to choose an arbi- trary profile. Grab an end of the profile line and move to the desired location on the matrix. The profiles are updated. D (30 Jul 2012) Data Acquisition Run JY Batch Experiments The Run JY Batch Experiments button runs a series of automated experiments, in- cluding adjustable repeats and delays between experiments. Click the Run JY Batch Experiments button The Setup batch experiments window appears. D (30 Jul 2012) Data Acquisition Select an experiment from the Execution List. In the Total Repeats: field, enter the number of times that experiment should be repeated. In the Delay before executing: field, enter the number of seconds to wait before executing. D (30 Jul 2012) Data Acquisition Real Time Control The Real Time Control button opens the Real Time Control window directly, so that the user can adjust experimental parameters in real time, while viewing effects of the adjustments. D (30 Jul 2012) Data Acquisition Set up the standards and unknown(s). If you have an automatic sample changer, you can change the samples automatically with the SC Auto radio button. For manual use with an automatic sample changer, choose SC Manual radio button, and the software prompts you to change the sample. D (30 Jul 2012) Data Acquisition Set up the excitation and emission wave- length(s). Enter the appropriate excitation and emission wavelength set(s), one per row, in the Wavelength Sets area. To add a wavelength set, click the Insert row button. The Calibration Curve Parame- ters window appears. Choose a Linear fit, or a Polynomial fit with Order of the polynomial, and then the OK button. D (30 Jul 2012) Data Acquisition 2D Intensity Map The 2D Intensity Map button creates a two-dimensional intensity map from the active microscope-mapping data. Note: This button only operates if data are displayed, and is used for microscope mapping purposes. D (30 Jul 2012) Data Acquisition Choose an excitation wavelength (row) to exam- ine. Here we have chosen 432 nm excitation. Click the 2D Intensity Map button 4-17. Note: This button only operates with TCSPC accessories. In the toolbar, click the Launch DataStation button. D (30 Jul 2012) Data Acquisition Switch menu between HJY Software Ap- plication and Origin Pro The Switch menu between HJY Software Application and Origin Pro.D (30 Jul 2012) Data Acquisition Multigroup Multigroup offers repeated and sequential fluorescence experiments.D (30 Jul 2012) Data Acquisition Connect to the desired instrument configuration. In the System Configuration area, select the configuration from the drop-down menu. Click the Connect Config button to initialize the instrument. Activate the excitation and emission monochromators’. D (30 Jul 2012) Data Acquisition Choose the slit- width parameters. Choose the detec- tor parameters.D (30 Jul 2012) Data Acquisition Running an unknown sample Often a researcher will scan a sample whose spectral characteristics are unknown. For optimal spectra, the optimal excitation and emission wavelengths must be found. The traditional method consists of running an emission scan to find the peak emission value. Choose integration time, increments, and number of scans judiciously, to give an accurate result without excessive time spent.D (30 Jul 2012) Data Acquisition Insert the sample into the sample compartment, and close the sample compartment’s cover. Click the Run button The scan starts.D (30 Jul 2012) Data Acquisition Set the scan parameters.D (30 Jul 2012) Data Acquisition Find the optimal emission peak.D (30 Jul 2012) Data Acquisition 4-30. D (30 Jul 2012) Optimizing Data 5: Optimizing Data Spectra can be enhanced by optimization of data-acquisition. This chapter lists some methods of optimizing sample preparation, spectrofluorometer setup, and data correc- tion to get higher-quality data. D (30 Jul 2012) Optimizing Data Dissolved solids Solid- sample Solid samples, such as crystals, sometimes holder are dissolved in a solvent and analyzed in solution. Solvents, however, may contain organic impurities that fluoresce and mask the signal of interest. D (30 Jul 2012) Optimizing Data Measuring the G factor Include the grating factor, or G factor, whenever polarization measurements are taken. The G factor corrects for variations in polarization wavelength-response for the emis- sion optics and detectors. D (30 Jul 2012) Optimizing Data Click the Detectors icon. This shows the parameters related to detectors, including the G factor, in the Polarization area. Click the G Factor checkbox to include a G fac- tor in your measurements. D (30 Jul 2012) Optimizing Data Improving the signal-to-noise ratio Because of various hardware or software conditions, occasionally it is necessary to op- timize the results of an experiment. This is true especially for weakly fluorescing samples with low quantum yields. D (30 Jul 2012) Optimizing Data Determining the optimum integration time The length of time during which photons are counted and averaged for each data point is referred to as the integration time. An unwanted portion of this signal comes from noise and dark counts (distortion inherent in the signal detector and its electronics when high voltage is applied). D (30 Jul 2012) Optimizing Data Using the appropriate wavelength increment The increment in a wavelength scan is the spacing, in nm, between adjacent data points. The spacing between the data points affects the resolution of the spectrum, and total time for acquisition. D (30 Jul 2012) Optimizing Data Selecting the appropriate bandpass The bandpass (wavelength spread) affects the resolution of your spectra. If the bandpass is too broad, narrow peaks separated by a small change in wavelength may be unresolved. D (30 Jul 2012) Optimizing Data Smoothing data Smoothing the data improves the appearance of the spectrum.In general, start with a 9- or 11-point smooth for a time-base measurement. To select the proper number of points for wavelength-scan types, first locate the area that re- quires smoothing—usually this is a peak. D (30 Jul 2012) Optimizing Data Correcting data Introduction Collecting accurate information about the fluorescent or phosphorescent properties of a sample depends upon several factors: Equipment specifications Sample characteristics Timing considerations. To ensure that the spectra collected indicate the actual properties of the sample and not external conditions, data often must be corrected. D (30 Jul 2012) Optimizing Data Choose Preferences, then the Instrument Correction Files icon. The Instrument Correction Files area should display a correction file for the Detector (S or R). If not, click the Insert button, and browse for the desired correction file. The corrected signal appears in the Formulas table. Run the experiment with the corrected signal. 5-17. D (30 Jul 2012) Optimizing Data After acquisition To apply the correction factors after the data have been acquired, multiply the data file by the appropriate correction factor file (mcorrect for the S detector or xcorrect for the R detector). D (30 Jul 2012) Optimizing Data The name of the chosen trace should appear in the Graph field. If not, browse for it with the drop-down menu. From Math Function, select multiply from the drop-down menu. D (30 Jul 2012) Optimizing Data 5-20. Replacing the lamp within the recommended time may prevent a catastrophic failure. Each time the lamp is turned on constitutes one full hour of use. Therefore, HORIBA Scientific suggests leaving the lamp on during brief periods of in- activity. D (30 Jul 2012) Maintenance Hazards Xenon-arc lamps are an explo- sion hazard. Wear explosion- proof face-shield and protective clothing when opening the lamp housing and handling the lamp. Disconnect the lamp power supply from the AC power line (mains) while handling lamp leads. D (30 Jul 2012) Maintenance Remove the lamp cover. With an Allen key, remove the five screws from inside the left wall of the sample compartment. Pull the lamp cover to the left about 2 Lift the cover vertically off. D (30 Jul 2012) Maintenance Remove the lamp-housing cover at the rear of the instrument. With a Phillips screwdriver, loosen the safety cover’s screw. Swing the safety cover out of the way. Remove the three cap screws from the lamp-housing cover. D (30 Jul 2012) Maintenance Rotate the cover backwards, and set it to the side of the instrument, so that electrical connections are not strained. The lamp is held in place by spring tension and the height adjust- ment on top of the. D (30 Jul 2012) Maintenance Press down against the spring action. Notice how the nipple faces away from the collection mirror (not visi- ble here behind the hand). Caution: Improper connections to the lamp severely affect lamp performance and affect the pow- er supply. D (30 Jul 2012) Maintenance Put the old lamp (in the top cover) in a safe place. Attach connections to the new lamp. Attach the cathode connection to the new Warning: Do not touch lamp, and secure the. D (30 Jul 2012) Maintenance Adjusting the new xenon lamp Choices There are two choices after lamp installation: Let the lamp “burn in”, i.e., run, for 6 h before adjustment of its position. Set the coarse lamp adjustments immediately. No further adjust- ment is necessary. Adjust the lamp: Continue with the Replace the lamp-housing cover.D (30 Jul 2012) Maintenance Acquire another water Raman scan. Use the same parameters as in step 3. Note the peak intensity. Use the flowchart below. Is the signal within speci- fications. No further adjustment is necessary. D (30 Jul 2012) Maintenance Electronics In case of the rare chance of system failure, this section is provided to help the user un- derstand the electronics components.D (30 Jul 2012) Maintenance Do not detach the screens’ screws. There is a J400906 control board underneath the instrument, which controls the drives, slits, shutter, and automated accessories in the monochromators. This board also con- trols options such as a sample changer. The console window appears, asking you to choose which device: Choose option 1 to update the FluoroMax-4 with USB port and current firmware version 1.00. The console asks you which choice: (1) update firmware, or (2) exit:. D (30 Jul 2012) Maintenance Choose 1 to update the firmware. The console asks you to confirm this choice: Enter Y to confirm updating the firmware. The console asks you to choose the firmware: 6-14. The software updates the firmware. When complete, the console prompts you to press any key to close the program: 6-15. If there is a problem, examine the chart below, and try the steps on the following pages. Problem Possible Cause Remedy. D (30 Jul 2012) Troubleshooting and self-absorption.No change in signal Detectors are saturated. Reduce slit settings. intensity. No signal. Lamp is not on. D (30 Jul 2012) Troubleshooting Hardware Init. Error Broken IR sensor in Replace IR sensor: Call the Ser- appears.Check status. D (30 Jul 2012) Troubleshooting Using diagnostic spectra Often the spectrum reveals information regarding the hardware or software parameters that should be adjusted. The following spectra occur with explanations about problems leading to their appearance. D (30 Jul 2012) Troubleshooting The following lamp-scan spectrum shows poor resolution in the area around the peak. 0.4473 Xenon-lamp peaks are unresolved 0.3355 0.2236 0.1118 Wavelength (nm) Poor lamp scan of 150-W Xe lamp. Note low resolution in the area near the 467-nm peak. D (30 Jul 2012) Troubleshooting Water Raman spectra Contaminated water Running a water Raman scan helps identify abnormalities caused by accessory prob- lems or miscalibration. The following spectrum is normal: Below is a normal water Raman spectrum superimposed on one that exhibits a problem. If the problem goes away, then the problem was due to the cuvette surface. Clean or use a different cuvette. D (30 Jul 2012) Troubleshooting Make sure that the excitation and emission slits are set to the proper widths. Stray light In the following diagram, notice the high level of stray-light below 380 nm in the wa- ter-Raman spectrum. D (30 Jul 2012) Troubleshooting Further assistance. Read all software and accessory manuals before contacting the Service Department. Of- ten the manuals show the problem’s cause and a method of solution. Technical support is available for both hardware and software troubleshooting. Click the View Sys- tem Info button. D (30 Jul 2012) Troubleshooting Determine the SpectrAcq firmware version: Open the Experiment Setup window: Click the Detectors icon Move the mouse over the detectors’ table in the Select area.D (30 Jul 2012) Troubleshooting 7-12. D (30 Jun 2012) Producing Correction Factors 8: Producing Correction Factors Introduction Gratings, detectors and other spectrometer components have response characteristics that are functions of wavelength. These characteristics are superimposed on spectra, and may yield a potentially misleading trace. HORIBA Scientific offers the F- 3026 Standard Lamp Accessory. The F-3026 Standard Lamp Assembly is a complete correction-factor kit, which in-. Enter the irradiance values into a spreadsheet. Save the file as IRR. D (30 Jun 2012) Producing Correction Factors Calculate the correction factors.D (30 Jun 2012) Producing Correction Factors Calculating emission correction factors Set up the F-3026 accessory. Attach the correction- factor light-source to its gap-bed with the brass thumbscrews. Use this orientation. Be sure the power cable doesn’t block optical apertures. D (30 Jun 2012) Producing Correction Factors Warning: Wear appropriate eye- protection against UV, visible, and IR when the tungsten- halogen lamp is on and the sam- ple-compartment is uncovered. Note: We recommend keeping a log of the amount of time the source is on. D (30 Jun 2012) Producing Correction Factors Analyze the data.D (30 Jun 2012) Producing Correction Factors Save this new file as irrad2.D (30 Jun 2012) Producing Correction Factors Turn OFF the acces- Caution: sory.Let the acces- sory cool before re- moval. Note: We recommend keeping a log of the amount of time the source is on. D (30 Jun 2012) Producing Correction Factors Choose Preferences. The Preferences area appears. Choose the Instrument Correction Files icon. The Instrument Correction Files area appears. If there are no active fields in the Instrument Correction Files area, click the Insert button. D (30 Jun 2012) Producing Correction Factors Then, in the File column, browse for the appropriate correction-factor file. When all necessary detectors have an associat- ed correction-factor file, click the OK button. Reinitialize the instrument and software. D (30 Jun 2012) Producing Correction Factors Excitation correction factors Excitation correction factors are measured during production of the instrument. If you have further questions, please contact the Service Department. 8-13. D (30 Jun 2012) Producing Correction Factors 8-14. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Theory of operation A second source of illumination, a pulsed xenon lamp, is used for phosphorescence measurements. Samples are excited with pulsed light; the emitted phosphorescence is measured using an R928P photon-counting detector. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation This sequence of excitation, delay, and sampling, is repeated for each lamp flash.D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation removes any interference from the lamp. Initial Delay can be varied with time to yield a decay curve. Spectra can be scanned to isolate different phosphorescing components based on the lifetime of the luminescent species in the sample. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Isolate components in a mixture based on lifetimes To the right are three scans of an aqueous mixture of terbium and europium chlo- rides that isolate different phosphorescent components based on their lifetimes. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Kinetic analysis of mixtures Often a sample containing a mixture of components can be analyzed through fitting its phosphorescence-decay curve.D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Operation of the phosphorimeter Start-up Load an appropriate instrument configuration that includes the phosphorimeter.D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Decay by These produce a decay of phosphorescence over time. Decay by Delay Delay varies the Delay after flash in order to construct the decay curve: Decay by Window Decay by Window varies the length of the Window increment with. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Processing phosphorimeter data Open the graph to be processed. Click on the data points to be processed. In the toolbar, choose Analysis. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Lamp replacement The xenon flash lamp typically has a half-intensity life of at least 10 million flashes.D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Hazards Xenon-arc lamps are an explosion hazard. Wear explosion-proof face-shield and protective clothing when opening the lamp housing and handling the lamp. Disconnect the lamp power supply from the AC power line (mains) while handling lamp leads. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Disconnect the RS-232 cable, power cord, and any other cables attached to the spectrofluorometer.D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Lift the cover vertically off the instrument. Remove the lamp-housing cover at the rear of the instrument. With a Phillips screwdriver, loosen the safety cover’s screw. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Remove the three Phillips screws from the lamp- housing cover. Gently rotate the lamp- housing cover with its cooling fans attached. Remove the flash lamp. D (30 Jul 2012) FluoroMax -4P Phosphorimeter Operation Insert the new flash lamp. Warning: Never touch the flash lamp’s glass bulb with bare hands. The oils from your hands can weaken the bulb and cause catastrophic failure. D (30 Jul 2012) Automated Polarizers 10: Automated Polarizers Introduction Theory The measurement of polarized emission of fluorescence allows the observation of rota- tional motions in fluorophores during the lifetime of the excited state. Because the rota- tion of macromolecules depends on their size, shape, and local environment (i.e., sol- vent), several kinds of information may be extracted. Polarization and anisotropy are expressed as follows: In a real optical system, the G, or grating factor, must be included to correct for the wavelength response to polarization of the emission optics and detectors. D (30 Jul 2012) Automated Polarizers Magic-angle conditions Some fluorescent compounds exhibit molecular rotations on the same time-scale as their fluorescent lifetimes. This can cause a spectral distortion if the excitation and emission channels of a spectrofluorometer show some polarization bias. New instrument and complete-polarizer or- ders are shipped with pre-aligned polarizers marked for excitation (“X”) or emission (“M”), and are locked in their collars. D (30 Jul 2012) Automated Polarizers Alignment Introduction Polarizer alignment is verified by measuring the anisotropy of a dilute scattering solu- tion. Scattered light is highly polarized, and this allows a simple check of the crystal alignment in the instrument. Open the Experiment Setup window. You may use any instrument configuration with polarizers. Click the Accessories icon. Click the Advanced. button. This opens the Polarizer Alignment window: 10-7. D (30 Jul 2012) Automated Polarizers Activate the Subtract Dark checkbox. Activate the Remeasure Anisotropy Only checkbox. Activate Reset to Mechanical Zero only if the polarizers are definitely miscalibrated.D (30 Jul 2012) Automated Polarizers Approve or retry the measurement based on satisfaction with the result. To quit, hit the Cancel button at any time during the procedure. As an alternative, use an Anisotropy scan to acquire the polarization (P) or anisotropy r ) to verify alignment. D (30 Jul 2012) Automated Polarizers Subtract Dark (recommended) Reset to Mechanical Zero—only if the polarizers are definitely miscalibrated. This deletes the previous calibration.D (30 Jul 2012) Automated Polarizers When complete, the software routine displays the measured anisotropy for the emission channel (S). Approve or retry the measurement based on satisfaction with the result.Depending on the accessories, the opportunity exists to remove polarization effects from the sample, measure the polarization characteristics, or analyze the decay of anisotropy using frequency-domain techniques. Real Time Control Real Time Control manipulates the polarizers and other instrument settings, to observe and optimize the spectrofluorometer in real time.