econo gradient pump manual
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econo gradient pump manualSince chromatography can be a time-consuming method, especially for preparative purification when larger volumes of sample (e.g. antibody purification from supernatant), washing or elution buffer are used, it is convenient to automate the process. Proteins can be sampled after isocratic or gradient elution with high accuracy to assure reproducibility.Several proteins needed to be purified from bacterial extracts for further identification as antigens via T cell assays and injection into rats. When we got the Econo Pump and accessories, assembly of the system, connection with our own fraction collector and chart reader, and calibration for the tube size, were easy to perform. This was due to the detailed instructions in the manual.This allowed us, after a few purifications, to analyze fewer fractions for the presence of our proteins of interest. Sometimes, when the urea buffer started to crystallize and clog the tubes, the fraction collector would stop or the built-up pressure would disconnect the tubes, so that the buffer and sample would spill onto the table. Therefore, I found that checking the tubes for crystallized urea and the connections for a tight fit, or exchanging tubes after several purifications to avoid leakage was important. We used the system continuously on a daily basis, but never experienced any other problems.Bio-Rad's website contains information about the pump combos, the accessories and tubes as well as the manuals. When the system arrives, you can call to receive assistance from a Bio-Rad representative in assembling the system.System can be assembled from the parts necessary for user-specific needs, making it possible to combine parts with already available devices e.g. fraction collector. Let us know! All rights reserved. Find products. Free subscription. To connect Econo UV Monitor or Econo Gradient Monitor to the Model 1327 Chart Recorder.http://www.ksaa.org.np/userfiles/cuisinart-ev-11pc9-manual.xml
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To connect both a Model 2128 Fraction Collector and Model 1327 Chart Recorder to the Econo Gradient Pump. To connect both a Model 2110 Fraction Collector and Model 1327 Chart Recorder to the Econo Gradient Pump. For more information on the BioLogic System family of products, contact your local Bio-Rad representative. Please check your inbox, and if you can’t find it, check your spam folder to make sure it didn't end up there. Please also check your spam folder. No user-serviceable parts are inside the unit. Refer servicing to Bio-Rad service personnel. Certified products are safe to use when operated in accordance with the instruction manual. This safety certification does not extend to other chromatography equipment or accessories not I.E.C. 1010 certified, even when connected to this Bio-Rad Econo Gradient Pump. It is possible that emissions from this product may interfere with some sensitive appliances when placed nearby or on the same circuit as those appliances. The user should be aware of this potential and take appropriate measures to avoid interference. Alteration of this instrument will void the manufacturer’s warranty, void the I.E.C. 1010 certification, and create a potential safety hazard for the user. It offers a full range of features to facilitate ease of use as a stand-alone pump or as a part of the BioLogic Duo-Flow System running software version 3.0 or later. The pump can be programmed to start or stop Bio-Rad fraction collectors and chart recorders. The EGP will store one program in its non-volatile memory. Remove the plastic bag and inspect the instrument for any external damage. Figure 2-1 shows the contents of the box. If any part is missing or damaged, contact your local Bio-Rad office immediately. Choices are shown A selection is indicated in uppercase letters. Pressing and holding a soft key and an arrow key acts to accelerate the action of the arrow keys.http://valdhans.cz/userfiles/cuisinart-filter-brew-coffee-maker-manual.xml A value input with the arrow key can be erased or accepted with the Cancel and Next keys respectively. Pressing and holding an arrow key will accelerate the action of the arrow keys. This allows The green and When Purge is pressed, The yellow Purge can be Advertissement:: Couper l'alimentation avant Simultaneous control of both the Model 2110 and the Model 1327, or the Model 2128 and the Model 1327, can be accomplished via the 2110-Y cable and the 2128-Y cable respectively.Tygon is suitable for most aqueous solutions. It will generally have the shortest lifetime of the three and should not be used for the pumphead. PharMed is the longest lasting of the three, and will provide the most consistent flow rate over time. Figure 4-1 shows approximate flow rate ranges with different tubing sizes.These items are included in the optional splitter valve kit. The PharMed tubing supplied with the Econo Gradient Pump has been cut to a predetermined length to accommodate tubing prestretch. (Silicon tubing in precut lengths is also available from Bio-Rad. See ordering information in Appendix D.) See below for the size and color of each lock-ring. Insert a barbed female luer fitting into the same end until the tubing reaches the flange of the fitting. Clamp the luer-fitting into place by sliding the lock ring along the tubing over the barbed fitting (see Figure 4-3). Repeat on the other end of tubing. My Bench Close Not a Member.You can adjust your Community Subscriptions in Settings Continue Removed. Features There are no Features available. General Specifications Part Number 7319001 Looking for New or Used Equipment. Shop on LabX Shop on Labx.com This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies. Please review our Privacy Policy for more details. All Rights Reserved. No user-serviceable parts are inside the unit. Remove the plastic bag and inspect the instrument for any external damage. Figure 2-1 shows the contents of the box.https://formations.fondationmironroyer.com/en/node/11717 Model EGP Econo Pump Low-Pressure Fittings Kit Power Cord System Tubing Pumphead Tubing Figure 2-1. Parts supplied with the Econo Gradient Pump. Different windows will appear on this display depending on the requests made by the user. Some windows will display operating parameters, while others will allow entry of variables such as flow rates, valve position and programming steps. Choices are shown in the lower row, above one of the three soft keys. A selection is indicated in uppercase letters. If no entry has been made, the previous window will be displayed. This allows passage through the windows in the reverse direction. The pump will run in the clockwise direction by factory default. When Purge is pressed, the pump will run at maximum speed (25 rpm), leaving the connected Gradient Mixer and Aux valve operating and the fraction collector and chart recorder off. The yellow purge LED is illuminated when purging. This key is not operable when the pump is running in Program mode. Advertissement:: Couper l'alimentation avant l'entretien et depannage. Silicone tubing, the most inert of the three, is suitable for aqueous and polar solutions. Tygon is suitable for most aqueous solutions. See below for the size and color of each lock-ring. Lightly pull the tubing around the rollers to remove slack. Attach the other end of tubing into the tubing retaining bracket on the opposite side of the pump head. 4. Slide the platen back into the pump head frame assembly until it rests up against the tubing. Press the cam lever in toward the pump head, locking the platen up against the tubing and rollers. Pressing the left or right soft keys will start the pump for the selected time. Collect the pump effluent in a graduated cylinder. Programming steps can be described in either units of time (in minutes) or volume (in ml). 6 Program Steps TIME vol Pressing the left soft key selects time; the right key selects volume.https://academyocgc.com/images/carestream-drx-1-manual.pdf Pressing Next advances to screen 8 if a Splitter valve is present or screen 9 if a Splitter valve is not present. Screen 7 (not displayed). This is available for future upgrades. Screen 8. Splitter Valve Period Selection. The Econo Gradient Pump can be connected to the BioLogic HR or BioLogic Duo-Flow workstation via an Instrument bus cable (System cables 17, 18, 19, or 21). When connected, the address of the Econo Gradient Pump must correspond to the address recognized by the BioLogic System. This address is only relevant when the Econo Gradient Pump is connected to the BioLogic System. The beeper, which beeps on each key press, can be disabled. 12 ON Beeper off Using the soft keys, select the desired option and press Next to accept and advance to screen 13. Screen 13. Erase Program Option. The Econo Gradient Pump will store one program in its non-volatile memory. The program stored in the Program mode can be erased by selecting the “yes” option. Press the Mode key until the Manual LED is illuminated. Up to three parameters (depending on how many optional devices are connected to the Econo Gradient Pump) can be controlled. Choose a field on the LCD by pressing the corresponding soft key. This option is available if a valve is connected to the Auxiliary port on back of the Econo Gradient Pump. The valve type is automatically recognized. The SV-3 diverter valve must be connected if you wish to program a collection window with either the model 2110 or 2128 Fraction collector. The arrow keys are then used to increment or decrement the value. Pressing the Step key or the Next key will accept the displayed values and advance to the next step. The program end occurs when either 6 steps have been entered, or two identical steps (pressing Step twice) are entered sequentially. If an auxiliary valve is present, a screen will appear after programming is completed. This will start the run. By using the right soft key, the Run screen can show time elapsed or time remaining on the run. If program steps are in volume, elapsed or remaining volume will also be displayed. Selecting Continue will continue the run. CONTINUE stop If Stop is selected, the program that was running is aborted. Pressing any key on the front panel will display the Manual mode. If Continue is selected, the program that was running is resumed at the point where the power failure occurred. If HOLD is selected during the program, then the pump will continue pumping at the current conditions and fraction collection will continue as programmed. If PAUSE is selected during the program, then the pump and fraction collector will stop. If the SV-3 diverter valve is connected to the EGP, then fraction collection will begin at the point in the program when the diverter valve is in the FRAC position. The pump and chart recorder will resume when CONTINUE is selected. Model EM-1 UV Monitor None A connection is not required between the pump and the EM-1 UV Monitor. It is supplied with the UV Monitor. To increase throughput, increase tubing size to obtain the desired output. Turn the socket such that the correct voltage shows through the window. 5. Reinsert the fuse drawer into the power entry module, with the locking tab to the left. Press gently until it snaps into place. 6. Insert the power cord plug into the power entry module. The LCD will display the program at the time of the power failure. Pressing the Stop soft key will display the message “Program Aborted”. You may now operate the pump as normal. Pump will not run Verify that power cord is plugged in and the power switch is turned on. Platen adjustment Adjust the platen by turning incorrect it counter clockwise until it is completely loosened, then rotate three to five full turns clockwise. See section 4.3, Platen Adjustment. Wrong tubing size Refer to Figure 4-1 for selection selected criteria. Select proper ID tubing within Setup.Pump is off Turn on the pump. These tasks are defined below. 9.1 FIRMWARE CONFIRMATION To check the version of firmware installed in the Econo Gradient Pump, power up the Econo Gradient Pump. During power up, the firmware version is displayed. Output signals are TTL compatible. To use these signals for non-Bio-Rad devices, you must insure that the circuit external to the Econo Gradient Pump does not draw more than 15 milliamperes of current. If any defects should occur during this warranty period, Bio-Rad will replace the defective parts without charge. Cables 731-8264 System Cable 4, 8-pin mini-DIN to banana cable. To connect Econo UV Monitor or Econo Gradient Monitor to the Model 1327 Chart Recorder. Other Features. Automatic rotor recognition, electronic imbalance detection, one-finger lid closure, soft brake function. Automatic rotor recognition, rotor recognition, electronic imbalance detection, safety lid lock. Biopharma industrial scale cell harvesting, broth clarification and separation of cell debris, separator can be a closed (sanitized) system, separates particles to 0.1 micronBe sure to use this value in calculating the rpms required for each rotor. Here are a few important guidelines for operating a centrifuge. These can help prevent damage to the centrifuge and rotor and more importantly prevent possible serious injury to you and others. Use the correct rotor and make sure it is properly installed. Make sure your work surface is level and firm. A centrifuge should never be in operation on an uneven work surface. Balance the load in a rotor. If it is an identical liquid that you are balancing against, then volume will work just fine. Stop or unplug a centrifuge if you see excessive shaking. Check to see if your tubes are balanced and that work surface is level. If that’s not the problem, schedule a service call. Don’t move or bump the centrifuge while it is spinning. Make sure the centrifuge is in a location where this cannot accidentally happen. Don’t open the lid to a centrifuge while it is spinning. Wear safety goggles if you are working near an operational centrifuge. Use the appropriate sample tubes for your rotor. Make sure your tubes are rated to be able to handle the max rcf of your rotor. Special tubes are needed for high force centrifugation.Limited. Yes. ProcessGrams to kilograms of purified compound. No. Proteins. Peptides. Sugars. NoReaction Speed. Standard. Detection. CCD camera. Scanning Photomultiplier Tube (PMT). Channel Photomultiplier (CPM) Fluorescence. Photodiodes. Excitation Source. Tungsten-Halogen LampDetects 1-10 copies of target sequence. Lid Design. Smart Lid (heated lid to minimize sample evaporation)Trademarks used herein are trademarks or registered trademarks of BioSurplus, Inc. All other names and brands are registered trademarks of their respective companies.Call Monday - Friday, 8am - 5pm, to talk with one of our scientific team members. Click here to Login or Register. By closing this banner or continuing to browse otherwise, you agree to the use of cookies. Find out more. The Econo gradient pump is a two-channel bi-directional pump that is ideal for preparative applications and for enhancing the purification of recombinant proteins (low-pressure chromatography). The dosing amount in time can be controlled when connected to the Model EP-1 Econo pump, the BioLogic LP system or the BioLogic DuoFlow system. The UV monitor is a dual wavelength detector for flow-through monitoring of chromatographic column effluents, centrifugation gradients and the like for liquid chromatography. Econo Gradient Pump in excellent condition. Packaged with tubes and all accessories. PicClick Exclusive You are the light of the world. However, the following defects are specifically excluded: 1. Defects caused by improper operation. 2. Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized agent. 3. Use with tubings or fittings not specified by Bio-Rad Laboratories for use with this system. 4. Deliberate or accidental misuse. 5. Damage caused by disaster. 6. Damage due to use of improper solvents or samples. 7. Damage due to spills. This warranty does not apply to tubing, fittings, and fuses. For inquiry or request for repair service, contact Bio-Rad Laboratories after confirming the model and serial number of your instrument.System components include a complete line of chromatography columns, prepacked cartridges, fittings, tubing, and valves. Each component has its own stand-alone features in addition to its role in the system. The Model ES-1 Econo System Controller integrates the individual com- ponents into a low pressure chromatography system. This manual contains the necessary information for setting up and 1 running the system. In some sections, references will be made to informati1 provided in the component manuals. For automated control of more than 2 buffers, the Model EV-1 Econo Buffer Selector must be connected to the Econo System controller. Aside from these requirements, UV and gradient monitors, frac- 2 tion collectors, and chart recorders from other manufacturers can be used with the system. Next, remove the system controller, by lifting under the base of the instrument. Do not pull on the ribbon cables. Carefully remove the plastic bag, and check for any obvious damage or problems with the instrument. Packed next to the instrument is a bag containing the connection cables, injector valve, and diverter valve. Figure 2.1 shows all the parts included with the Econo System controller. Check off all parts against the sup- plied packing list. Other components of the Automated Econo System (Model EP-1 Econo Pump, Model EM-1 Econo UV Monitor, etc.) are packaged sep- arately. If any parts are missing or damaged, contact Bio-Rad Laboratories imme- diately. The gra- dient LED display gives, quick identification of gra- dient progression. Display Function Fraction Collector Output This 8-pin mini-DIN sock- et is used for sending an advance signal to the Model 2110 Fraction Collector vta Econo System cable 1. Diverter Valve Output Chart Recorder Output 7 This 8-pin mini-DIN sock- et sends a signal to the diverter valve, thus divert- ing flow from a fraction collector or collection device to another recepta- cle (waste), or vice versa. This 8-pin mini-DIN sock- et is used for connection of the Model 1325 or Model 1327 Econo Chart Recorder. This connector is also used for connecting non-Econo System components including fraction collec- tors, UV monitors, and chart recorders to the Econo System. See Appendix A for details. 3.3 Topside Functions Dynamic Mixer Gradient \ Prop. Valve Fig. 3.3 Top view of the Econo System controller. 8 Display Gradient Proportioning Valve Mixer Ribbon Cables Function This valve is used for pro- portioning two solvents for the purpose of forming lin- ear gradients, as dictated by the Gradient Former control. Dynamic mixer for the rapid mixing of two solutes. These 34- and 40-pin rib- bon connectors are for con- necting the Model EM-I Econo UV Monitor and Model EP-1 Econo Pump, respectively, to the Econo System controller. Power is supplied to the system controller through the 40- pin Econo Pump connec- tor. Section 4 Econo System Controller Connections and Set Up The Econo System can be set up in a variety of locations, including 4 oc cold rooms or cold boxes. The orientation of the system is up to the user. The Econo System Rack is designed to hold the Model EM-1 Econo UV Monitor optics module, diverter valve, and sample injection valve. The Econo System Organizer is designed to lift up the Econo System Controller for placement of the Model EG-1 Econo Gradient Monitor and Model EV-1 Econo Buffer Selector underneath. For this reason, it is recommended that the Econo Rack and Econo System Organizer be set up before proceeding. Ribbon cables on the top of the system controller are used for connection to the underside of the pump and UV monitor.Install the female 1uer fittings by inserting the barbed end of the fitting into the tubing, and clamp the luer fitting into place by sliding the lock-ring over the barbed fitting (see Figure 5.4). Repeat on the other end of the tubing. 16 Affix male luer fittings to both ends as described above, and connect to the female luer fittings on both the pump outlet and the mixer. If a Model EV-1 Econo Buffer Selector is to be used, do not connect the mixer to the pump. Continue with Section 5.4 for plumbing the Model SV-5 Valve Pod. Fig. 5.4. Attachment of tubing fittings. 5.4 Valve Pod The Model SV-5 Valve Pod should be positioned as close as possible to the mixer on the Econo System controller and the Econo Pump. The length of tubing should be of minimal length and inner diameter. Buffers A and B should already be plumbed through the proportioning valve and mixer on the Econo System Controller. Connect tubing from the outlet of the mixer on the Econo System controller to the AlB inlet port on the Model SV-5 Valve Pod. Two methods for manual sample injection are described below. One uses preset injection volumes with a classical injection loop sys- tem, post-pump; the other allows pre-pump variable injection volumes. The MV-6 injector valve can be used in both configurations. Alternatively, a three-way valve can be inserted in-line between the pump and the column inlet or between the pump and mixer to provide a simple injection port. For details on automated sample injection, see Section 7.8. 18 Post-Pump Injection with a Sample Loop 1. The Model MV-6 Injector Valve can be installed in a variety of loca- tions on the rack. Use the screw fitting on the injector valve housing to secure the valve. 2. Cut a section of tubing long enough to connect the pump and the injec- tor port. Affix male luer fittings as described above. 3. An injection loop of a desired volume can be formed by using a section of tubing of a length which corresponds to a desired volume. Table 5.1 provides the tubing lengths that correspond to several common loop volumes. 4. Attach a male luer-fitting to each end of the sample loop tube. 5. Connect the inlet line from the pump, sample loop, and outlet line to the column as shown in Figure 5.4. Arrow indicates rotation direction of valve. The tubing end used to connect the outlet of the chro- matography column should have a female luer fitting attached. Arrows on the top of the optics module are used to indicate flow direction. The tubing ends to be connected to the inlet and outlet lines of the UV monitor should be affixed with a male luer fitting. It is important to minimize the length and inner diameter of this section of tubing. 20 3-way Stopcock Connect the Model EG-1 Econo Gradient Monitor flow cell directly to the inlet or outlet port of the UV optics module for optimal conductivity detection. Warning: Do not overtighten the luer fittings. 5. 7 Model SV-3 Diverter Valve The Model SV-3 Diverter Valve should be coupled as close as possible to the UV monitor outlet. The length of tubing used should be of minimal length and inner diameter. Connect two sections of tubing with a male luer fitting on one end and connect to both the collect and waste ports of the diverter valve. Direct the tubing from the waste port to an appropriate waste container. Connect the tubing from the collector port to a fraction collector or suitable collection device as described in Section 5.8. 5.8 Fraction Collector To connect the diverter valve to the Model2110 Fraction Collector, affix a male luer fitting to one end of a length of tubing, and attach this connector to the female luer fitting to the collection port of the flow diverter valve. Place the other end of the tubing into the rubber ferrule of the fraction collector drop forming arm. See the Model 2110 Fraction Collector instruction man- ual for details on this connection. Minimizing the length of tubing used for this connection will improve the accuracy with which peaks are collected. Section 6 Getting Started After the Econo System is connected and plumbed, it can be powered up. It is important that the input voltage match the voltage on the power entry mod- ule. Refer to Section 2.2 if it does not match. 6.1 Connecting System to Power The following line cords need to be connected to power up the system. 1. Model EP-1 Econo Pump 2. Model EM-1 Econo UV Monitor 3. Model EV-1 Econo Buffer Selector (requires power adaptor) 4. Model EG-1 Econo Gradient Monitor (requires power adaptor) 5. Model 2110 Fraction Collector 6. Model 1325 or Model 1327 Econo Recorder (optional, both require power adaptor) 21 The Model EP-1 Econo Pump will supply the power to the system con- troller through the ribbon cable connected to the pump base. The Model EM-1 Econo UV Monitor is plugged directly into a grounded power source, as are the gradient monitor, buffer selector, chart recorder and fraction collector. The UV monitor is powered up with the lamp on. Ensure that the optics module is plugged into the appropriate socket on the rear panel of the UV monitor. The UV lamp LED will flash during the first minute or two. Allow at least 15 minutes for the UV lamp, and 10 minutes for the gradient monitor to warm up. 6.2 System Check Before proceeding, it is advisable to check the system for leaks or other problems. To carry out the test, place buffer lines for buffer A and B into a reservoir containing water. Disconnect any chromatography column during the test. When the valve cycles between waste and collect, a click should be heard. If not, check the connection with the system controller. Using the scroll keys on the pump, increase the pump output to 25. The pump head should begin to tum. Over the next 10 minutes, watch for any leaks or air bubbles. Restart the pump. 22 5. 6. 7. Section 7 Press the Purge key on the pump front panel and let pump operate at 100 maximum output (25 rpm) of buffer A for several minutes. Again, check for any leaks in the system. When purging, the diverter valve should be in the waste position. To prime with 100 buffer B, press the Gradient Former key (see Figure 3.1) while in the Purge mode. Note: To prime the buffer lines for buffers C, D, and E (if the Econo Buffer Selector is being used), the system must be configured in the Enhanced mode (See Section 7.1). Buffers A through E can then be selected by depressing the Gradient Former key. Release the key when the desired buffer is displayed on the Econo Buffer Selector display. The valves will not change until the key is released.For more spe- cific details on the operation of the individual components, refer to the man- uals supplied with them. This section summarizes the function of the system in various separation strategies, and various levels of complexity. 7.1 Getting Started: Verification of Econo System Software Version The Model EP-1 Econo Pump must have software version 2.01 or high- er to control the Model EV-1 Econo Buffer Selector. Call your local representative for an EP-1 ROM Replacement Kit. Disconnect the Econo Pump from all connections and replace the ROM fol- lowing the directions in the ROM Replacement Kit. The ROM replacement procedure takes about 15 minutes. If you do not wish to perform the ROM replacement, please contact your local Bio-Rad representative. If your pump currently features software version 2.01 or higher, or if the ROM upgrade has already been completed, ensure that all connections between the Econo Pump, Econo System Controller and Econo Buffer Selector have been completed and the system power is on. 7.2 Econo System Software Mode Selection Software version 2.01 and higher features two user-selectable program- ming modes, Standard and Enhanced. The system will remain in the mode selected until a different mode is selected (even after power-down.) The Standard and Enhanced modes have separate memories. The pump will power up in the Stop mode, and the UV monitor with the lamp on. See the UV monitor manual for details. 4. When using the system in an isocratic fashion, make sure that solvent line A is being used, and is properly connected to the proportioning valve. See the pump manual for detailed calibra- tion procedures. To stop flow, press once more. Flow rate will be displayed in mllmin. Change the flow to the desired speed while the pump is running by pressing the arrow keys. Simply press the key to light the indicator light below the figure corresponding to either waste or collect. The default position of the valve is in the waste position. Tip: The system can be programmed for automatic shut-off of the iso- cratic run. This can be done by programming pump will shut off once the total run volume a total has run been volume delivered. (V1). The For detail, see Tip in the following section. Time Based Collection with the Model 211 0 Fraction Collector To enable the Model 2110 Fraction Collector: 1. Connect the fraction collector to the system con- troller or Model EP-1 Econo Pump using Econo System Cable 1. 2. Press the Fraction Collector key on the Model EP-1 Econo Pump front panel to enter the fraction col- lector edit mode. In the edit mode, only the flashing keys are active. Note that the Fraction Size and ml indicator lights are flashing, prompting entry of frac- tion size. Enter the fraction size in 0.1 ml increments using the Arrow keys. After the fraction size is entered, press the Fraction Collector key once more to confirm the fraction size. 3. Enter a the Arrow void volume keys. See (V the 0 ) Model in 1 ml increments using EP-1 Econo Pump manual for details on tion.