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cytofluor ii manualOther Features. Automatic rotor recognition, electronic imbalance detection, one-finger lid closure, soft brake function. Automatic rotor recognition, rotor recognition, electronic imbalance detection, safety lid lock. Biopharma industrial scale cell harvesting, broth clarification and separation of cell debris, separator can be a closed (sanitized) system, separates particles to 0.1 micronBe sure to use this value in calculating the rpms required for each rotor. Here are a few important guidelines for operating a centrifuge. These can help prevent damage to the centrifuge and rotor and more importantly prevent possible serious injury to you and others. Use the correct rotor and make sure it is properly installed. Make sure your work surface is level and firm. A centrifuge should never be in operation on an uneven work surface. Balance the load in a rotor. If it is an identical liquid that you are balancing against, then volume will work just fine. Stop or unplug a centrifuge if you see excessive shaking. Check to see if your tubes are balanced and that work surface is level. If that’s not the problem, schedule a service call. Don’t move or bump the centrifuge while it is spinning. Make sure the centrifuge is in a location where this cannot accidentally happen. Don’t open the lid to a centrifuge while it is spinning. Wear safety goggles if you are working near an operational centrifuge. Use the appropriate sample tubes for your rotor. Make sure your tubes are rated to be able to handle the max rcf of your rotor. Special tubes are needed for high force centrifugation.Limited. Yes. ProcessGrams to kilograms of purified compound. No. Proteins. Peptides. Sugars. NoReaction Speed. Standard. Detection. CCD camera. Scanning Photomultiplier Tube (PMT). Channel Photomultiplier (CPM) Fluorescence. Photodiodes. Excitation Source. Tungsten-Halogen LampDetects 1-10 copies of target sequence. Lid Design.http://g-stampo.com/pics/pages/epson-eb-s17-manual.xml
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Smart Lid (heated lid to minimize sample evaporation)Filter-based optical system for emission and excitation. Excitation filters: 360, 450, 485, 530, 590. Emission filters: 460, 530, 580, 620, 645 Filters: Trademarks used herein are trademarks or registered trademarks of BioSurplus, Inc. All other names and brands are registered trademarks of their respective companies.Call Monday - Friday, 8am - 5pm, to talk with one of our scientific team members. Click here to Login or Register. By closing this banner or continuing to browse otherwise, you agree to the use of cookies. Find out more. Not for use in diagnostic procedures. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document. ABI PRISM, Applied Biosystems, BioCAD, CytoFluor, GeneScan, INTEGRAL, POROS, Procise and Tropix are registered trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries. AB (Design), Applera, Biospectrometry, CytoCalc, Data Explorer, Expedite, FMAT, Mariner, Pioneer, Proteomics Solution 1, SPRINT, VISION, and Voyager are trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries. ICAT is a trademark of the University of Washington in the U.S. and certain other countries, exclusively licensed to Applied Biosystems Group of Applera Corporation. MDS is a registered trademark and SCIEX is a trademark of MDS, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Microsoft, MS, Windows, and MS-DOS are registered trademarks of Microsoft Corporation. IBM is a registered trademark of International Business Machines Corporation.http://customoid.co.uk/userfiles/epson-eb-s92-manual.xml Attophos is a trademark of JBL Scientific. BODIPY, PicoGreen, Texas Red, SNARF, and SNAFL are registered trademarks of Molecular Probes, Inc. AlamarBlue is a registered trademark of AccuMed International, Inc. All other trademarks are the sole property of their respective owners. A WARNING For continued protection against fire hazard, replace fuses with those of the same type and rating. AVERTISSEMENT Remplacez les fusibles par ceux de meme type et puissance pour eviter les risques d’incendie. It is an ETL Testing Laboratories listed product. EMC This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Warning: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. Note: Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits. Canadian Safety and EMC (Electromagnetic Compliance) Standards Safety This instrument has been tested to and complies with standard C22.2 No. 1010-1-92, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use; Part 1: General Requirements”. C’est un produit homologue par les ETL Testing Laboratories. EMC This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations. Cet appareil numerique de la classe A respecte toutes les exigences du Reglement sur le materiel brouilleur du Canada.Controlling the Temperature. Minimum Computer Requirements (You Supply). Optical Sets for Fluorophores. CytoFluor Standard Filter Sets. Ordering Information. 8.1 Specifications 8.2 8.3 8.4 8.5 8-2 8-3 8-4 8-6 8-7 Appendix A Introduction to Windows. A-1 Appendix B Error Messages. C-4 Appendix D Plate Scan Patterns. D-1 Appendix E Technical Support and Training. This system enables you to scan data on soluble and cell-associated fluorescence. The software runs on a PC that you provide and enables you to control and maintain the scanner. Audience This manual is written for CytoFluor system users. You should be familiar with using a PC and Windows applications. For your convenience, there is a glossary of terms at the back of this document. If you need more information on Windows, refer to the manuals provided with the Windows software. Structure of this guide Applied Biosystems CytoFluor Series 4000 Multi-Well Fluorescence Reader User’s Guide is divided into chapters. Each chapter page is marked with a tab and a header to help you locate information within the chapter. The table below describes the material covered in each chapter. Chapter 1, Overview of the CytoFluor System Provides an overview of the CytoFluor system process. Chapter 2, Getting Started Describes how to get started. For example, it defines the function of the scanner parts and describes how to set it up. It also contains steps to install the software and check the system. CytoFluor User’s Guide xi 1 How to Use This Guide xii Applied Biosystems Chapter 3, Scanning Operations Defines how to create a new file, access a previously saved file, and set up the scan parameters. It also contains details on how to scan a plate. Chapter 4, Displaying, Printing, and Exporting Data Contains steps to use post processing for data display. It also describes how to print and export data. Chapter 5, Setting the Plate Layout or Auto Scale Parameters Provides details on how to set the plate layout. For example, it defines how to assign characteristics to a plate well or group of wells. It also describes how to set the auto scale parameters so the system can scale the data as it acquires it. Chapter 6, Customizing the CytoFluor System Describes how to customize the system. It includes steps to set up and change filter pairs, test the scanner drives and lamp, and set the lamp saver timer. Chapter 7, Maintaining the CytoFluor System Defines how to maintain the CytoFluor system. For example, it describes how to clean the scanner and change the air filters. It also has steps to change the lamp when needed. Chapter 8, Accessing Technical Information Contains details on the system specifications and catalog numbers. Appendix A, Introduction to Windows Provides basic information on how to use Microsoft Windows and defines basic Microsoft Windows terms. 1 Conventions General Conventions Notes, Cautions, and Warnings Appendix B, Error Messages Describes and defines CytoFluor system test vectors and error messages. Appendix D, Plate Scan Patterns Provides examples of the scan patterns the CytoFluor system uses when averaging points taken in a well. Appendix E, Technical Support and Training Describes how to contact Technical Support, obtain technical documents, and obtain customer training information. Notes, Cautions, and Warnings are used for the following reasons: NOTE: Informs the user about important information not critical to operating the system. CAUTION Warns the user about possible harm to the system or loss of data. CytoFluor User’s Guide xiii 1 How to Use This Guide WARNING Warns the user about possible bodily harm. AVERTISSEMENT Un avertissement fournit une information indispensable a la securite de l’operateur. Symbols used on the system The following symbol appears on the system to alert the user to warnings. The symbol also appears next to associated warnings in this document. Le symbole ci-dessous permet d’alerter l’utilisateur et precede un avertissement. Send us your comments xiv Applied Biosystems We welcome your comments and suggestions for improving our manuals. Applied Biosystems must receive your completed installation qualification report before you can receive technical support and product update information. The registration number for your system software is on the program disk. CytoFluor User’s Guide 1-1 1 Chapter 1 Overview of the CytoFluor System 1.1 What is the CytoFluor System. The fluorescence is measured by a photomultiplier tube in a light-proof detection chamber. The detected fluorescence data is displayed as arbitrary fluorescence units (AFU). You control the action of the scanner through the software. The software runs on a PC operating under Microsoft Windows software. The precision X-Y mechanism scans the microtiter plate with a sensitive fiber-optic probe to quantify the fluorescence in each well. The plate remains stationary during the scanning of each row, then indexes to the next row. The scanning system is fast; it can scan a 96-well plate in under a minute. You can use standard types of microtiter plates with six to 384 wells. You can select plate formats from a library of plates or define your own plate format as described in Chapter 5, Setting the Plate Layout or Auto Scale Parameters. For plates with larger wells, you can choose an option to obtain an average of multiple data points in each well. (Appendix D, Plate Scan Patterns, defines the acquisition patterns.) In addition, you can configure the system to read plates from the top or bottom, depending on your application. (See Chapter 6, Customizing the CytoFluor System, for more information.) The scanner holds up to six excitation filters and six emission filters to measure most common fluorophores. The CytoFluor system ships with five excitation and five emission filters installed. The remaining positions are filled with light tight plugs. (See Chapter 8, Accessing Technical Information, for a list of common fluorophores and their corresponding filters.) In addition, the scanner requires little maintenance. Controlling the temperature provides the ability to hold a plate at a specific temperature or collect data from kinetic experiments. TR models can be upgraded with a temperature control chamber. Contact Applied Biosystems for more information. CytoFluor User’s Guide 1-3 1 Chapter 1 Overview of the CytoFluor System 1.2 How Does It Work? The CytoFluor software uses a Microsoft Windows interface to provide features that make the system easy to use. For example, you select commands and options from menus and buttons using the mouse. If you prefer, use keyboard equivalents to access many of the functions. The software initially displays the CytoFluor main setup screen. This screen contains the scan set up parameters and menu options. You can access options to assign plate parameters to reflect your actual assay plate and experimental setup. You can also access options to save, copy, or print your data. (Chapter 2, Getting Started, contains more information on the CytoFluor main setup screen.) The scan setup parameters include identification information, excitation and emission filter pairs, gain, number of scans and cycles, and other run-specific information. You can edit them at any time, before you acquire data. Once you acquire data, you can only change the comments information in a file. You start collecting data by selecting the Start button from the main setup screen after you enter the scan setup information. The software directs the scanner to move the plate into the detection chamber. The scanner mechanism then aligns the probe to read all selected wells, row by row. A run for a single plate may have up to 200 cycles, with up to four different filter pairs and sensitivities per cycle. Each group of filter pairs and gain represents a scan. After the system reads the final well, the plate transport mechanism presents the plate back to the load position. The detector is set to a low gain setting and the lamp remains on to minimize warm-up time for successive runs. The system has a timer that turns the lamp and detector off if no runs have been initiated for 60 minutes. You can change this value using the Maintenance menu as described in Chapter 6, Customizing the CytoFluor System. The system stores scanned information and the setup in a CytoFluor data file. This involves setting the voltage, installing fuses, and connecting it to a PC. Chapter 2, Getting Started 2 Load the software, check the system functions, and run system diagnostics. Chapter 2, Getting Started 3 Set or check the scan parameters of a file. Or copy the parameters of an existing file and rename it. Chapter 3, Scanning Operations 4 Determine the plate layout to identify particular wells or groups of wells before starting or completing a run (optional). Chapter 5, Setting the Plate Layout or Auto Scale Parameters 5 Set the auto scale parameters so the system can scale the data as it acquires it (optional). Chapter 5, Setting the Plate Layout or Auto Scale Parameters 6 Customize the system before a run (optional). Chapter 6, Customizing the CytoFluor System 7 Insert a loaded plate and perform a run. Chapter 3, Scanning Operations 8 Display, print, or export data. Chapter 4, Displaying, Printing, and Exporting Data CytoFluor User’s Guide 1-5 1 1 Chapter 1 Overview of the CytoFluor System Step 9 Action Maintain the CytoFluor scanner. After you unpack the parts, review this list to make sure you are not missing anything: Part CytoFluor Scanner NOTE: See Section 2.2, The CytoFluor Scanner, for details. CytoFluor system software System Calibration Certificate NOTE: It identifies the scanner and software by serial number. See Appendix E, Technical Support and Training, for the phone number. 2-4 Applied Biosystems The CytoFluor Scanner 2 2.2 The CytoFluor Scanner The scanner contains all the parts necessary to scan a variety of plates. Once you connect the scanner to your computer and load the CytoFluor software, you can control how it functions. You can also determine the way the scanner reads the plate wells and customize it to suit your needs. See Chapter 5, Setting the Plate Layout or Auto Scale Parameters, and Chapter 6, Customizing the CytoFluor System, for more details. This section defines how each scanner part functions. See Section 2.4, Setting Up Your System, for information on how to set up the scanner. Front of the scanner Figure 2-1 shows the front of the scanner. CytoFluor User’s Guide 2-5 2 Chapter 2 Getting Started Inside the scanner To see the inside parts, open the scanner cover by turning the thumbwheel counterclockwise and lifting up the cover. To replace the cover: Make sure the plate carrier is towards the rear panel so you do not close the cover on it. Make sure the fiber-optic cables are inside the scanner. Then pull the cover over the base, push it down, and turn the thumbwheel clockwise until finger-tight. CAUTION Be sure to seat the cover properly so the plate carrier can exit the scanner. After you follow the steps in Section 2.4.4, Installing the Software, click on the Plate In and Plate Out buttons to test the plate carrier. If it does not move in and out, reseat the cover. Figure 2-2 shows the inside of the scanner. D C G F C A E B Figure 2-2 Inside of Scanner NOTE: In CytoFluor Series 4000 scanners with temperature control, the plate carrier is contained in a temperature control chamber with an additional door. These features are not shown. 2-6 Applied Biosystems The CytoFluor Scanner 2 Letter Part Name Function A Plate carrier Holds the plate so the scanner can read the well contents. B Emission filter assembly Holds the filter that determines the fluorescence emission wavelength to monitor. C Probe Transmits and receives the excitation and emission signal. D Top read mounting block Holds the probe so it can read from the top of the plate. E Bottom read mounting block Holds the probe so it can read from the bottom of the plate. F Excitation filter assembly Holds the filter that selects the excitation wavelength. G Lamp Provides the fluorescent excitation. (The lamp is located at the rear of the scanner in a separate, covered compartment.) CytoFluor User’s Guide 2-7 2 Chapter 2 Getting Started Back of the scanner Figure 2-3 shows the back of the scanner. C F A E D B PB100531 Figure 2-3 Back of Scanner Letter Part Name Function A Air vent Draws air into the scanner. B RS-232 port Enables you to connect the scanner to a computer. D Power switch Enables you to turn the scanner on and off. E Power inlet Enables you to connect the power cord. F Fuse module Enables you to change the fuses. 2-8 Applied Biosystems Computer System Requirements 2.3 Computer System Requirements You supply the computer to use with the CytoFluor system. WARNING For continued protection against fire hazard, replace fuses with those of the same type and rating. AVERTISSEMENT Remplacez les fusibles par ceux de meme type et puissance pour eviter les risques d’incendie. 2-10 Applied Biosystems Setting Up Your System Installing 2 To install the fuses: 1. Remove the warning label from the power module. 2. Remove the fuse module by inserting a small flathead screwdriver in the slot beside the power plug and sliding out the fuse module (Figure 2-4). Slot Power module Fuse module PB100496 Figure 2-4 Removing the Fuse Module 3. Install the 2.5 amp fuses in the fuse module (Figure 2-5). Install IEC-approved fuses in all other countries. 2.5 amp fuses Fuse module PB100493 Figure 2-5 Installing the Fuses CytoFluor User’s Guide 2-11 2 Chapter 2 Getting Started 4. Slide the fuse module into the power module until it snaps into place (Figure 2-6). Power module Fuse module PB100492 Figure 2-6 Replacing the Fuse Module 5. Install the power cord. 2-12 Applied Biosystems Setting Up Your System 2 2.4.2 Removing the Shipping Restraints To remove the shipping restraints: 1. Open the scanner cover by turning the thumbwheel counterclockwise and lifting up the cover. Thumbwheel Figure 2-7 Opening the Cover 2. Remove the shipping restraints from the probe and transport assemblies shown in Figure 2-8. Cut the tie wraps with a small wire cutter. NOTE: CytoFluor Series 4000 Temperature Control models do not have a shipping restraint at position 1 (Figure 2-8). You must, however, remove a piece of tape from the temperature control chamber door. CytoFluor User’s Guide 2-13 2 Chapter 2 Getting Started Shipping restraint position 1 Tie wraps Rails Tie wrap PB100532 Figure 2-8 Tie Wraps 3. Inspect the rails to ensure they are clean. Clean them with ethanol or isopropanol if necessary. 4. Close the scanner cover, push it all the way down to seat it, and turn the thumbwheel clockwise until finger-tight. CAUTION Do not over-tighten or under-tighten the cover thumbwheel or the plate carrier cannot exit the scanner. To make sure you closed the cover correctly, click Plate Out. If the plate carrier does not move out, turn off the scanner and turn the thumbwheel further to tighten it. Thumbwheel Figure 2-9 Closing the Cover 2-14 Applied Biosystems Setting Up Your System 2 2.4.3 Connecting the Scanner to Your Computer After you install fuses in the scanner, and you remove the shipping restraints, you can connect the scanner to your computer. WARNING You must electrically ground the system and computer. These components are equipped with grounding plugs. Use the grounding plugs in a grounded outlet. Using a grounded plug that has been converted to a non-grounded plug can cause electric shock or system damage. AVERTISSEMENT Pour les connexions electriques, vous devez mettre le systeme et l'ordinateur a la terre. Ces composants sont equipes de fiches de mise a la terre. Utilisez ces dernieres avec une prise de courant reliee a la terre. L'utilisation d'une fiche de mise a la terre, transformee en fiche non-protegee peut causer des secousses electriques ou endommager le systeme. CytoFluor User’s Guide 2-15 2 Chapter 2 Getting Started To connect the scanner to your computer: 1. Plug the RS-232 cable into Communication Port 1 or 2 on the computer. Make a note of the port you used to connect the scanner. Figure 2-10 Connecting the RS-232 Cable to the Computer 2. Connect the other end of the RS-232 cable into the Serial Port (9-pin connector) on the rear of the scanner. The Run dialog box appears. The default destination path is C:\CytoFlr. 2-18 Applied Biosystems Setting Up Your System 2 The Setup Complete dialog box appears when the installation is complete. Figure 2-14 Installation Complete Dialog Box 6. Select Yes, Launch the program file and click on Finish to bring up the CytoFluor main setup screen: Figure 2-15 CytoFluor Main Setup Screen CytoFluor User’s Guide 2-19 2 Chapter 2 Getting Started You can now check the system as described in the Section 2.5, Checking the System. If you have problems installing the software, contact Applied Biosystems Technical Support. See Appendix E, Technical Support and Training, for the phone number. Displaying the software version 2-20 Applied Biosystems To check the version of the installed software, open the About Cytofluor dialog box. To do so, select About Cytofluor from the Help menu. Checking the System 2 2.5 Checking the System After you complete the set up steps, you must check the CytoFluor system before you can use it. CytoFluor User’s Guide 2-21 2 Chapter 2 Getting Started The CytoFluor main setup screen appears: Figure 2-16 CytoFluor Main Setup Screen Chapter 3, Scanning Operations, contains details on how to use this screen to set run parameters. 4. 2-22 Applied Biosystems Access the Maintenance screen as described in Section 2.5.2, Accessing the Maintenance Screen. Checking the System 2 2.5.2 Accessing the Maintenance Screen To check your scanner settings, use the Maintenance screen. To access this screen: 1. Pull down the Maintenance menu (or press ALT, M, and Enter). NOTE: You can use key equivalents to choose options instead of clicking with the mouse. The CytoFluor software shows the key equivalent as an underscored letter in the name of an option. For example, Maintenance has the key “M” as its equivalent. Press ALT, M, Enter to choose it. The Maintenance screen appears: Figure 2-17 CytoFluor Maintenance Screen 2. See Section 2.5.3, Checking Scanner Settings, to check the scanner settings. CytoFluor User’s Guide 2-23 2 Chapter 2 Getting Started 2.5.3 Checking Scanner Settings To check the scanner settings: 1. Check the filter settings shown in the Excitation Filters and Emission Filters fields of the Maintenance screen. The CytoFluor system ships with five excitation and five emission filters. You may add or replace filters by following the steps in Section 6, Customizing the CytoFluor System. 2. Check the Port to Reader selection. If you have more than one serial port on your computer, note the name of the port where you physically connected the scanner. See step 3 to change the setting. 3. Click on the down arrow next to the Port to Reader field to scroll down a list of port selections. Highlight the computer port to which you configured the CytoFluor system and release the mouse to select it. 4. See Section 2.5.4, Running System Diagnostics, to run system diagnostics. NOTE: Keep a dated record of the settings. Update the record when filters are changed or added. 2.5.4 Running System Diagnostics To run system diagnostics: 1. Click the Test button on the right side of the Maintenance screen. The CytoFluor software automatically checks the scanner drives and lamp. The test takes several seconds. When testing is completed, the Unit Test dialog box appears (Figure 2-18). 2-24 Applied Biosystems Checking the System 2 Figure 2-18 Unit Test Dialog Box If all systems pass, the scanner is ready for use. If the Lamp Status fails, then replace the lamp. See “Replacing the Lamp” on page 7-5 for detailed instructions. If any other system fails, contact Applied Biosystems Technical Support. See Appendix E, Technical Support and Training, for the phone and fax numbers. 2. Click Print to print a CytoFluor Fluorescence Unit Test Report. 3. Click OK to close the box. 2.5.5 Returning to the Main Setup Screen If you have been working with the software, follow these steps to return to the main setup screen: 1. Click the Save button to keep any changes you made before returning to the main setup screen. Or, click Cancel to discard any changes you made. CytoFluor User’s Guide 2-25 2 Chapter 2 Getting Started 2. 2-26 Applied Biosystems Begin working with the system. Continue to Chapter 3, Scanning Operations, for details on how to perform scans. Or, shut down the system as described in Section 2.6, Shutting Down the System. Shutting Down the System 2 2.6 Shutting Down the System To shut down the system: 1. Select Exit from the File menu (ALT, F). 2. Exit Windows. 3. Power down the scanner and computer. CytoFluor User’s Guide 2-27 2 Chapter 2 2-28 Getting Started Applied Biosystems Scanning Operations 3 3 This chapter includes the following sections: 3.1 Creating a New File. 3-2 3.2 Setting Scan Parameters. 3-7 3.3 Setting Up Multiple Scans in a Cycle. 3-16 3.4 Saving Scan Parameters. 3-20 3.5 Controlling Temperature. 3-22 3.6 Scanning Plates. 3-24 CytoFluor User’s Guide 3-1 3 Chapter 3 Scanning Operations 3.1 Creating a New File Once you start up the CytoFluor system as described in Chapter 2, Getting Started, you can create a new file. To do this, you have several options. See this chart for details: You can. Enter new settings and create a new file name. For instructions. See Section 3.1.1, Creating a New, Original File. NOTE: If you do not have an existing file in the system, follow the steps in this section to create one. Access the data of a preexisting file to adopt the settings of that file See Section 3.1.2, Accessing a Previously Saved File. Set the scan parameters through the CytoFluor main setup screen before naming the file. See Section 3.2, Setting Scan Parameters. NOTE: The scan parameters enable you to enter information unique to the experiment. You can select the run mode, mix time, reads per well, and so on. See Section 3.2, Setting Scan Parameters, for details. You can save a file and enter a new file name before or after you set the scan parameters. See the following sections for details. 3-2 Applied Biosystems Creating a New File 3 3.1.1 Creating a New, Original File If you want to clear the data of an existing file or set the parameters of a new file, follow these steps: 1. Start up CytoFluor as described in Chapter 2, Getting Started. You see the option menus and main setup screen. 2. Pull down the File menu and choose New (ALT, F, N). The system changes all the plate data to zero (if present); the scan parameters remain unchanged. 3. See Section 3.2, Setting Scan Parameters, for details on how to set new parameters.